You are here: GulfBase > Projects & Programs > Identification of Oyster Genes Differentially Expressed After Exposure to Mercury Using Microarray Technology	•

	Home Upcoming Events Institutions & Organizations People Bays & Estuaries Reefs, Banks & Islands Environmental Issues General Facts about the Gulf Exploration History Other Online Resources Electronic Books Mexican Coral Reef Species Checklist InfoHub Site Search:		Identification of Oyster Genes Differentially Expressed After Exposure to Mercury Using Microarray Technology In this project, we propose to develop microarray technoloy for monitoring of biological impact of environmental pollution. Oyster cDNA microtechnology will be developed for study of genomic expression signatures (GES) in response to environmental agents to identify genees affected by mercury, under both acute and more subtle, long-term exposures. Biological effects of mercury on survival, growth, and reproduction will be studied in relation to the genomic expression signatures. cDNA microarrays will be developed by using normalized and subtracted cDNA clones. The microarrays will be comparatively hybridized to probes developed from samples before and after treatment of mercury. Pollutant-specific genomic expression signatures will be identified, characterized, and developed into genechips as highly sensitive and reliable tools for monitoring of biological impact of environmental pollution. The cDNA microarrays will provide advanced technological tools for study of other environmental stresses by changing the treatment compound and offer highly sensitive and reliable indicators for monitoring marine environment. The project outputs include normalized cDNA libraries made from oysters with all mRNAs being "equally" represented; genechips containing large numbers of expressed sequence tag clones representing a large part of the genomic expression; identified genes induced by exposure to mercury. These are marine biotechnological reagents needed to address marine environmental problems using biotechnology, and can be applicable to address any other environmental problem. A significant part of the output will be the advances in marine environmental science and coastal ecosystem health assessment through use of genechip technology. Pollutant-specific and dose-dependent promoters can be isolated in the near future from the affected genes, which will be the basis for studies of in situ bioindication. Long-term benefits relate to the development of a new generation of technologies based on environmental genomics. Principal Investigator(s) or Program Chair: Liu, Zhanjiang Sponsor(s): Sea Grant Mississippi-Alabama Institution(s): Auburn University Field of Focus: Oyster gene identification following mercury exposure Study Site:   Mississippi Sound Award Period:   2001 - 2004 E-mail:   zliu@acesag.auburn.edu Keywords:   Oyster, Gene, Identification, Mercury, Microarray, cDNA, Microtechnology, GES
			Protected Area: Change your Password Edit your GulfBase Record Forgot your Username or Password?

GulfBase is a project of the Harte Research Institute for Gulf of Mexico Studies at Texas A&M University-Corpus Christi. To give proper credit to the original authors, please cite information taken from GulfBase by the original source as displayed. To cite GulfBase, use: M. Nipper, J.A. Sánchez Chávez, and J.W. Tunnell, Jr., Editors. 2010. GulfBase: Resource Database for Gulf of Mexico Research. World Wide Web electronic publication. http://www.gulfbase.org, 09 February 2010.

You are visitor since October 15, 2002.	GulfBase Team   Contact Info